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plasmid pbad24- t4rnl1  (GenScript corporation)

 
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    GenScript corporation plasmid pbad24- t4rnl1
    (A) Schematic overview of Hi-GRIL-seq and iRIL-seq workflows. WT (XL2001) and the hfq R16A mutant (FDM1050) were transformed with either an empty vector (pBAD24) or a <t>T4</t> <t>RNA</t> Ligase I-expressing vector (pBAD24- t4rnl1 ). Biological duplicates of each strain were cultured in LB (Lennox) with ampicillin at 37°C until reaching an OD600 of ∼0.5, at which point arabinose was added to induce enzyme expression. Cells were then grown to early stationary phase (OD600 ∼1.0), harvested, and processed for either Hfq IP followed by RNA isolation (iRIL-seq) or direct RNA extraction for total RNA sequencing (Hi-GRIL-seq). sRNAs are shown in orange, mRNAs in black, tRNA in green and rRNA in blue. This figure was created in BioRender ( https://BioRender.com/jqqlsju ). (B) Circos plots illustrating RNA interactomes in WT and R16A Hfq with either pBAD24 or pBAD24- t4rnl1 , as detected by iRIL-seq. (C) Quantification of S-chimeras under different conditions (Supplementary Table S11). Table displaying the number of RNA pairs with significant chimeric fragments identified by iRIL-seq, with values shown for two biological replicates and their calculated mean. (D) Chimera levels of specific RNA pairs identified by RIL-seq (anti-Hfq) and iRIL-seq. The RNA pairs shown here include the six pairs analyzed in previous experiments as well as four additional pairs identified as having significant numbers of chimeras in the iRIL-seq R16A experiment. Chimera counts were extracted from Supplementary Table S6 (RIL-seq, anti-Hfq) and Supplementary Table S11 (iRIL-seq), using the same method described in . For each RNA pair, the values represent the mean from two biological replicates.
    Plasmid Pbad24 T4rnl1, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pbad24-+t4rnl1/bio_rxiv__2025__06__26__660752-47-1-12?v=GenScript+corporation
    Average 90 stars, based on 1 article reviews
    plasmid pbad24- t4rnl1 - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "RNA-RNA Interactome Approaches Provide in vivo Evidence for a Critical Role of the Hfq Rim Face in sRNA-mRNA Pairing"

    Article Title: RNA-RNA Interactome Approaches Provide in vivo Evidence for a Critical Role of the Hfq Rim Face in sRNA-mRNA Pairing

    Journal: bioRxiv

    doi: 10.1101/2025.06.26.660752

    (A) Schematic overview of Hi-GRIL-seq and iRIL-seq workflows. WT (XL2001) and the hfq R16A mutant (FDM1050) were transformed with either an empty vector (pBAD24) or a T4 RNA Ligase I-expressing vector (pBAD24- t4rnl1 ). Biological duplicates of each strain were cultured in LB (Lennox) with ampicillin at 37°C until reaching an OD600 of ∼0.5, at which point arabinose was added to induce enzyme expression. Cells were then grown to early stationary phase (OD600 ∼1.0), harvested, and processed for either Hfq IP followed by RNA isolation (iRIL-seq) or direct RNA extraction for total RNA sequencing (Hi-GRIL-seq). sRNAs are shown in orange, mRNAs in black, tRNA in green and rRNA in blue. This figure was created in BioRender ( https://BioRender.com/jqqlsju ). (B) Circos plots illustrating RNA interactomes in WT and R16A Hfq with either pBAD24 or pBAD24- t4rnl1 , as detected by iRIL-seq. (C) Quantification of S-chimeras under different conditions (Supplementary Table S11). Table displaying the number of RNA pairs with significant chimeric fragments identified by iRIL-seq, with values shown for two biological replicates and their calculated mean. (D) Chimera levels of specific RNA pairs identified by RIL-seq (anti-Hfq) and iRIL-seq. The RNA pairs shown here include the six pairs analyzed in previous experiments as well as four additional pairs identified as having significant numbers of chimeras in the iRIL-seq R16A experiment. Chimera counts were extracted from Supplementary Table S6 (RIL-seq, anti-Hfq) and Supplementary Table S11 (iRIL-seq), using the same method described in . For each RNA pair, the values represent the mean from two biological replicates.
    Figure Legend Snippet: (A) Schematic overview of Hi-GRIL-seq and iRIL-seq workflows. WT (XL2001) and the hfq R16A mutant (FDM1050) were transformed with either an empty vector (pBAD24) or a T4 RNA Ligase I-expressing vector (pBAD24- t4rnl1 ). Biological duplicates of each strain were cultured in LB (Lennox) with ampicillin at 37°C until reaching an OD600 of ∼0.5, at which point arabinose was added to induce enzyme expression. Cells were then grown to early stationary phase (OD600 ∼1.0), harvested, and processed for either Hfq IP followed by RNA isolation (iRIL-seq) or direct RNA extraction for total RNA sequencing (Hi-GRIL-seq). sRNAs are shown in orange, mRNAs in black, tRNA in green and rRNA in blue. This figure was created in BioRender ( https://BioRender.com/jqqlsju ). (B) Circos plots illustrating RNA interactomes in WT and R16A Hfq with either pBAD24 or pBAD24- t4rnl1 , as detected by iRIL-seq. (C) Quantification of S-chimeras under different conditions (Supplementary Table S11). Table displaying the number of RNA pairs with significant chimeric fragments identified by iRIL-seq, with values shown for two biological replicates and their calculated mean. (D) Chimera levels of specific RNA pairs identified by RIL-seq (anti-Hfq) and iRIL-seq. The RNA pairs shown here include the six pairs analyzed in previous experiments as well as four additional pairs identified as having significant numbers of chimeras in the iRIL-seq R16A experiment. Chimera counts were extracted from Supplementary Table S6 (RIL-seq, anti-Hfq) and Supplementary Table S11 (iRIL-seq), using the same method described in . For each RNA pair, the values represent the mean from two biological replicates.

    Techniques Used: Mutagenesis, Transformation Assay, Plasmid Preparation, Expressing, Cell Culture, Isolation, RNA Extraction, RNA Sequencing



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    plasmid pbad24- t4rnl1  (GenScript corporation)
    90
    GenScript corporation plasmid pbad24- t4rnl1
    (A) Schematic overview of Hi-GRIL-seq and iRIL-seq workflows. WT (XL2001) and the hfq R16A mutant (FDM1050) were transformed with either an empty vector (pBAD24) or a <t>T4</t> <t>RNA</t> Ligase I-expressing vector (pBAD24- t4rnl1 ). Biological duplicates of each strain were cultured in LB (Lennox) with ampicillin at 37°C until reaching an OD600 of ∼0.5, at which point arabinose was added to induce enzyme expression. Cells were then grown to early stationary phase (OD600 ∼1.0), harvested, and processed for either Hfq IP followed by RNA isolation (iRIL-seq) or direct RNA extraction for total RNA sequencing (Hi-GRIL-seq). sRNAs are shown in orange, mRNAs in black, tRNA in green and rRNA in blue. This figure was created in BioRender ( https://BioRender.com/jqqlsju ). (B) Circos plots illustrating RNA interactomes in WT and R16A Hfq with either pBAD24 or pBAD24- t4rnl1 , as detected by iRIL-seq. (C) Quantification of S-chimeras under different conditions (Supplementary Table S11). Table displaying the number of RNA pairs with significant chimeric fragments identified by iRIL-seq, with values shown for two biological replicates and their calculated mean. (D) Chimera levels of specific RNA pairs identified by RIL-seq (anti-Hfq) and iRIL-seq. The RNA pairs shown here include the six pairs analyzed in previous experiments as well as four additional pairs identified as having significant numbers of chimeras in the iRIL-seq R16A experiment. Chimera counts were extracted from Supplementary Table S6 (RIL-seq, anti-Hfq) and Supplementary Table S11 (iRIL-seq), using the same method described in . For each RNA pair, the values represent the mean from two biological replicates.
    Plasmid Pbad24 T4rnl1, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pbad24-+t4rnl1/bio_rxiv__2025__06__26__660752-47-1-12?v=GenScript+corporation
    Average 90 stars, based on 1 article reviews
    plasmid pbad24- t4rnl1 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic overview of Hi-GRIL-seq and iRIL-seq workflows. WT (XL2001) and the hfq R16A mutant (FDM1050) were transformed with either an empty vector (pBAD24) or a T4 RNA Ligase I-expressing vector (pBAD24- t4rnl1 ). Biological duplicates of each strain were cultured in LB (Lennox) with ampicillin at 37°C until reaching an OD600 of ∼0.5, at which point arabinose was added to induce enzyme expression. Cells were then grown to early stationary phase (OD600 ∼1.0), harvested, and processed for either Hfq IP followed by RNA isolation (iRIL-seq) or direct RNA extraction for total RNA sequencing (Hi-GRIL-seq). sRNAs are shown in orange, mRNAs in black, tRNA in green and rRNA in blue. This figure was created in BioRender ( https://BioRender.com/jqqlsju ). (B) Circos plots illustrating RNA interactomes in WT and R16A Hfq with either pBAD24 or pBAD24- t4rnl1 , as detected by iRIL-seq. (C) Quantification of S-chimeras under different conditions (Supplementary Table S11). Table displaying the number of RNA pairs with significant chimeric fragments identified by iRIL-seq, with values shown for two biological replicates and their calculated mean. (D) Chimera levels of specific RNA pairs identified by RIL-seq (anti-Hfq) and iRIL-seq. The RNA pairs shown here include the six pairs analyzed in previous experiments as well as four additional pairs identified as having significant numbers of chimeras in the iRIL-seq R16A experiment. Chimera counts were extracted from Supplementary Table S6 (RIL-seq, anti-Hfq) and Supplementary Table S11 (iRIL-seq), using the same method described in . For each RNA pair, the values represent the mean from two biological replicates.

    Journal: bioRxiv

    Article Title: RNA-RNA Interactome Approaches Provide in vivo Evidence for a Critical Role of the Hfq Rim Face in sRNA-mRNA Pairing

    doi: 10.1101/2025.06.26.660752

    Figure Lengend Snippet: (A) Schematic overview of Hi-GRIL-seq and iRIL-seq workflows. WT (XL2001) and the hfq R16A mutant (FDM1050) were transformed with either an empty vector (pBAD24) or a T4 RNA Ligase I-expressing vector (pBAD24- t4rnl1 ). Biological duplicates of each strain were cultured in LB (Lennox) with ampicillin at 37°C until reaching an OD600 of ∼0.5, at which point arabinose was added to induce enzyme expression. Cells were then grown to early stationary phase (OD600 ∼1.0), harvested, and processed for either Hfq IP followed by RNA isolation (iRIL-seq) or direct RNA extraction for total RNA sequencing (Hi-GRIL-seq). sRNAs are shown in orange, mRNAs in black, tRNA in green and rRNA in blue. This figure was created in BioRender ( https://BioRender.com/jqqlsju ). (B) Circos plots illustrating RNA interactomes in WT and R16A Hfq with either pBAD24 or pBAD24- t4rnl1 , as detected by iRIL-seq. (C) Quantification of S-chimeras under different conditions (Supplementary Table S11). Table displaying the number of RNA pairs with significant chimeric fragments identified by iRIL-seq, with values shown for two biological replicates and their calculated mean. (D) Chimera levels of specific RNA pairs identified by RIL-seq (anti-Hfq) and iRIL-seq. The RNA pairs shown here include the six pairs analyzed in previous experiments as well as four additional pairs identified as having significant numbers of chimeras in the iRIL-seq R16A experiment. Chimera counts were extracted from Supplementary Table S6 (RIL-seq, anti-Hfq) and Supplementary Table S11 (iRIL-seq), using the same method described in . For each RNA pair, the values represent the mean from two biological replicates.

    Article Snippet: The T4 RNA Ligase I-expressing plasmid pBAD24- t4rnl1 was synthesized by the GenScript company.

    Techniques: Mutagenesis, Transformation Assay, Plasmid Preparation, Expressing, Cell Culture, Isolation, RNA Extraction, RNA Sequencing